Flow Cytometry

Group Head: Alessio Palini, Ph.D. Contact


The Flow Cytometry Core is a resource platform offering state-of-the-art instrumentation and analysis techniques to the scientific community. Flow cytometry is an evolving field used by scientists to answer a multitude of questions in their discovery work. The primary goal of the core is to support researchers and the other cores in obtaining verifiable results for such applications as immunophenotyping, cell division and apoptosis, cell activation, intracellular pH shifts, phagocytosis, oxidative burst and many more. Because of the wide-ranging applicability of this technology and in line with the NIHS inter-disciplinary environment, a “Translational Cytometry” approach is required to “bridge” basic research and clinical protocols based on nutritional intervention for which cytometry analysis is essential. In addition to the analytical capabilities of this technique, the core facility offers assisted cell sorting services for characterising, separating and purifying populations of particles as diverse as beads, bacteria, micro-particles, cells and chromosomes.

Key Goals

  • Provide specifically designed immunophenotyping panels and flow cytometric techniques for innovation in targeted fields of nutrition and diagnostics.
  • Introduction of new and sophisticated analyzers, cell sorters and analysis techniques allowing for more rapid and complex multiparametric analyses (up to 22 parameter analysis) thus providing greater correlation of analytes, efficiency and a reduction of overall costs.
  • Implementation of a new technology that will bridge the gap between high-speed cell sorting and extremely rare event recovery. This technology called the DEP Array (Di-electrophoretic cell caging process) permits the subsequent identification and recovery of extremely rare post-sorted cells for transcriptome analysis by the genomics core.

 

Key Publications of the Group*

Boss C, De Marchi U, Hermant A, Conrad M, Sizzano F, Palini A, Wiederkehr A, Bouche N. Encapsulation of Insulin-Secreting Cells Expressing a Genetically Encoded Fluorescent Calcium Indicator for Cell-Based Sensing In Vivo. Adv Healthc Mater. 2017 Feb;6(4).

Wang J, Mauvoisin D, Martin E, Atger F, Galindo AN, Dayon L, Sizzano F, Palini A, Kussmann M, Waridel P, Quadroni M, Dulić V, Naef F, Gachon F. Nuclear Proteomics Uncovers Diurnal Regulatory Landscapes in Mouse Liver. Cell Metab. 2017 Jan 10;25(1):102-117.

Ammirati E, Moroni F, Magnoni M, Di Terlizzi S, Villa C, Sizzano F, Palini A, Garlaschelli K, Tripiciano F, Scotti I, Catapano AL, Manfredi AA, Norata GD, Camici PG. Circulating CD14+ and CD14highCD16- classical monocytes are reduced in patients with signs of plaque neovascularization in the carotid artery. Atherosclerosis. 2016 Dec;255:171-178.

Lukjanenko L, Jung MJ, Hegde N, Perruisseau-Carrier C, Migliavacca E, Rozo M, Karaz S, Jacot G, Schmidt M, Li L, Metairon S, Raymond F, Lee U, Sizzano F, Wilson DH, Dumont NA, Palini A, Fässler R, Steiner P, Descombes P, Rudnicki MA, Fan CM, von Maltzahn J, Feige JN, Bentzinger CF. Loss of fibronectin from the aged stem cell niche affects the regenerative capacity of skeletal muscle in mice. Nat Med. 2016 Aug;22(8):897-905. See news release

Ammirati E, Magnoni M, Moroni F, Di Terlizzi S, Scotti I, Villa C, Sizzano F, Impellizzeri M, Fanelli G, Esposito G, Chiesa R, Camici PG. Reduction of Circulating HLA-DR+ T Cell Levels Correlates With Increased Carotid Intraplaque Neovascularization and Atherosclerotic Burden. JACC Cardiovasc Imaging.2016.

Ferrari G, Rabiolo A, Bignami F, Sizzano F, Palini A, Villa C, Rama P. Quantifying Ocular Surface Inflammation and Correlating It With Inflammatory Cell Infiltration In Vivo: A Novel Method. Invest Ophthalmol Vis Sci.2015;56(12):7067-75.

*Some of these publications were done before the scientist/s joined the Nestlé Institute of Health Sciences